Modifications in a Reference Freeze-Dried Direct Agglutination Test to Improve Visceral Leishmaniasis Detection
نویسندگان
چکیده
منابع مشابه
Leish-KIT, a stable direct agglutination test based on freeze-dried antigen for serodiagnosis of visceral leishmaniasis.
In order to increase the application potential of the direct agglutination test (DAT) for the detection of anti-Leishmania antibodies in human serum samples, we developed an antigen based on stained and freeze-dried Leishmania donovani promastigotes. We describe here the evaluation of the performance of the DAT based on this freeze-dried antigen. It was shown that the freeze-dried antigen remai...
متن کاملSeroepidemiological study of visceral Leishmaniasis (human infection) in East Myankooh area, in Lorestan Province by Direct Agglutination Test (DAT)
Background: Leishmaniasis is an infection caused by a protozoan of the genus Leishmania. This descriptive and cross-sectional study was done in order to determine the seroepidemiology of visceral leishmaniasis in East Myankooh area, in Lorestan Province during 2004 – 2005 time period. Materials and methods: This study was a descriptive, cross-sectional study. Multi stage sampling was performed...
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Background: Visceral leishmaniasis (VL) is an endemic disease in some parts of Iran. Many techniques have been used for diagnosis of VL, among which the urine based la-tex agglutination test (KAtex) is a promising one. Objective: To compare three diag-nostic tests of VL including KAtex, ELISA and Direct Agglutination Test (DAT) in VL patients and healthy controls in the south west of Iran. Meth...
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The objectives of this study were to compare the sensitivities and reliabilities of direct agglutination test (DAT), indirect immunofluorescent antibody test (IFAT), polymerase chain reaction (PCR) and bone marrow aspiration for the diagnosis of Mediterranean visceral leishmaniasis (MVL) using blood samples. Blood samples were collected from 67 confirmed MVL patients and 47 patients with other ...
متن کاملDirect agglutination test with urine samples for the diagnosis of visceral leishmaniasis.
A new direct agglutination test (DAT) for use with urine samples for the diagnosis of visceral leishmaniasis (VL) has been developed and compared with the conventional DAT with serum samples and our previously reported enzyme-linked immunosorbent assay (ELISA) with urine samples (urine ELISA). The new DAT, in which anti-human IgG was used as enhancing antibody, was tested with urine samples fro...
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ژورنال
عنوان ژورنال: The American Journal of Tropical Medicine and Hygiene
سال: 2020
ISSN: 0002-9637,1476-1645
DOI: 10.4269/ajtmh.19-0745